Imod ctfphaseflip
Witryna1 gru 2009 · CTFPHASEFLIP corrects images line-by-line by inverting phases appropriately in thin strips of the image at nearly constant defocus. CTF correction by these methods is shown to improve the resolution of aligned, averaged particles extracted from tomograms. ... This paper has described methods implemented in … WitrynaIMOD_TMPDIR Path for temporary directory used by scripts. On Windows, this can be in Cygwin or Windows format, with forward or back slashes. IMOD_USE_GPU IMOD_USE_GPU2 These variables will tell the Tilt and Ctfphaseflip programs to use the GPU on a CUDA-capable NVIDIA graphics card. Set the variable to 0 to use the best …
Imod ctfphaseflip
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WitrynaDirectives for Batch Processing and Template Files. Must be final extension; required in the -directive entry to Etomo. Overrides value from scanHeader; applies to B axis unless brotation used. First part of bidirectional tilt series is in second part of file for A or only axis. First part of bidirectional tilt series is in second part of file ... Witryna4 lut 2024 · The CTF was estimated using GCTF 61 and then the IMOD ctfphaseflip implementation was used for phase correction 62. Tomograms were manually segmented using AMIRA (Thermo Fisher Scientific) or ...
WitrynaChanges for IMOD 4.11. Changes marked with ** are particularly noteworthy, ones marked with * may also be of interest to typical users. ... * 10/22/18: Ctfphaseflip is … Witryna1 paź 2009 · The CTF was estimated using GCTF 61 and then the IMOD ctfphaseflip implementation was used for phase correction 62. B Nearest neighbor distance …
WitrynaIMOD - Boulder Laboratory for 3-D Electron Microscopy of Cells - IMOD/ctfphaseflip.cpp at master · LabShare-Archive/IMOD Witryna1 lut 2024 · Defocus measurements were done using IMOD's Ctfplotter, ... (CTF) correction done by phase inversion using IMOD's Ctfphaseflip. Tomographic reconstructions by weighted back-projection were done using IMOD's Tilt, while SIRT reconstructions were produced using Tomo3D (Agulleiro and Fernandez, 2011). The …
WitrynaStripe-based CTF correction (with ctfphaseflip from Imod) Creation of tomograms with Dynamo; These preprocessing operations that go from the tilt series to the tomograms can be used for any sample. Tomogram management. This part is specific to the particular geometry of the proteins of interest. Cataloguing the tomograms (archiving)
Witryna1 wrz 2024 · The 2D strip-based approach performed using IMOD’s ctfphaseflip is slower than the simple angle-independent CTF correction used in NovaCTF for a … christ church cathedral nelsonWitrynaIMOD - Boulder Laboratory for 3-D Electron Microscopy of Cells - IMOD/ctfplotter.preman at master · LabShare-Archive/IMOD christ church cathedral nashville episcopalWitryna25 wrz 2024 · Defocus estimation was performed using IMOD ctfplotter , phase flipping was performed using NovaCTF or ctfphaseflip, and dose weighting was performed using IMOD mtffilter. Tomograms were aligned using IMOD etomo and subsequently reconstructed using NovaCTF, with the exception of eight tomograms that were used … geometry symmetry quizletWitryna19 maj 2024 · CTF correction: CTF correction of a set of input tilt-series using the IMOD procedure. More info: ctfphaseflip doc; Dose filter: Tilt-series dose filtering based on … geometry syllabus 10th gradeWitryna5 lis 2024 · Tomograms were reconstructed in IMOD using weighted back-projection, with a simultaneous iterative reconstruction technique (SIRT)–like filter to aid visualization and segmentation. Defocus-contrast tomograms were contrast transfer function corrected in IMOD using ctfphaseflip. VPP tomograms were left uncorrected. geometry syllabus 9th gradechrist church cathedral nelson new zealandWitryna1 lip 2012 · Local approaches, however, are currently the standard in the field. For example the program CTFPHASEFLIP [6] as incorporated in the widely used software package IMOD [16] ... Projection after (local) tilted phase flipping using CTFPHASEFLIP, which is part of IMOD. (D) Difference between (B) and (C). The tilt-axis is horizontal. … geometry syllabus doc